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A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

Authors :
Ina Ersing
Luis Nobre
Liang Wei Wang
Lior Soday
Yijie Ma
Joao A. Paulo
Yohei Narita
Camille W. Ashbaugh
Chang Jiang
Nicholas E. Grayson
Elliott Kieff
Steven P. Gygi
Michael P. Weekes
Benjamin E. Gewurz
Source :
Cell Reports, Vol 19, Iss 7, Pp 1479-1493 (2017)
Publication Year :
2017
Publisher :
Elsevier, 2017.

Abstract

Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

Details

Language :
English
ISSN :
22111247
Volume :
19
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.6d63a6d1609541e78227973b624273aa
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2017.04.062