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Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry

Authors :
Danye Qiu
Miranda S. Wilson
Verena B. Eisenbeis
Robert K. Harmel
Esther Riemer
Thomas M. Haas
Christopher Wittwer
Nikolaus Jork
Chunfang Gu
Stephen B. Shears
Gabriel Schaaf
Bernd Kammerer
Dorothea Fiedler
Adolfo Saiardi
Henning J. Jessen
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

Myo-Inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) are important second messengers but their analysis remains challenging. Here, the authors develop a capillary electrophoresis-mass spectrometry method for the identification and quantitation of InsP and PP-InsP isomers in cells and tissues.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.6c9f68025794db3aaf128b31c15130b
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-19928-x