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Active DNA unwinding and transport by a membrane-adapted helicase nanopore

Authors :
Ke Sun
Changjian Zhao
Xiaojun Zeng
Yuejia Chen
Xin Jiang
Xianting Ding
Lu Gou
Haiyang Xie
Xinqiong Li
Xialin Zhang
Sheng Lin
Linqin Dou
Long Wei
Haofu Niu
Ming Zhang
Ruocen Tian
Erica Sawyer
Qingyue Yuan
Yuqin Huang
Piaopiao Chen
Chengjian Zhao
Cuisong Zhou
Binwu Ying
Bingyang Shi
Xiawei Wei
Ruotian Jiang
Lei Zhang
Guangwen Lu
Jia Geng
Source :
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Publication Year :
2019
Publisher :
Nature Portfolio, 2019.

Abstract

Active translocation of DNA through nanopores usually needs enzyme assistance. Here authors present a nanopore derived from helicase E1 of bovine papillomavirus (BPV) which acts as a conductive pore embedded in lipid membrane to allow the translocation of ssDNA and unwinding of dsDNA.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
10
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.6b935d4164446e5bff1e992c773483b
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-019-13047-y