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Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Authors :
Pei-Heng Lin
Sheng-Cih Huang
Kuang-Po Chen
Bor-Ran Li
Yaw-Kuen Li
Source :
Sensors, Vol 19, Iss 1, p 28 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA–containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm2) with a strong preference being exhibited for shorter aniline-based linkers.

Details

Language :
English
ISSN :
14248220 and 19010028
Volume :
19
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Sensors
Publication Type :
Academic Journal
Accession number :
edsdoj.6b33db2989b3495a8e858afd3574c879
Document Type :
article
Full Text :
https://doi.org/10.3390/s19010028