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Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables

Authors :
Rongqi Zhai
Ge Chen
Guangyang Liu
Xiaodong Huang
Xiaomin Xu
Lingyun Li
Yanguo Zhang
Donghui Xu
A. M. Abd El-Aty
Source :
Foods, Vol 12, Iss 1, p 196 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Imidacloprid is one of the most commonly used insecticides for managing pests, thus, improving the quality and yield of vegetables. The abuse/misuse of imidacloprid contaminates the environment and threatens human health. To reduce the risk, a colorimetric enzyme-linked immunoassay assay (Co-ELISA) and chemiluminescence enzyme-linked immunoassay assay (Cl-ELISA) were established to detect imidacloprid residues in vegetables. The linear range of Co-ELISA ranged between 1.56 μg/L and 200 μg/L with a limit of detection (LOD) of 1.56 μg/L. The values for Cl-ELISA were 0.19 μg/L to 25 μg/L with an LOD of 0.19 μg/L, which are lower than those of Co-ELISA. Fortifying Chinese cabbage, cucumber, and zucchini with imidacloprid at 10, 50, and 100 μg/L yielded recoveries between 81.7 and 117.6% for Co-ELISA and at 5, 10, and 20 µg/L yielded recoveries range from 69.7 to 120.6% for Cl-ELISA. These results indicate that Cl-ELISA has a high sensitivity and a rapid detection time, saving cost (antigen and antibody concentrations) and serving as a more efficient model for the rapid detection of imidacloprid residue.

Details

Language :
English
ISSN :
23048158
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Foods
Publication Type :
Academic Journal
Accession number :
edsdoj.697a599b0a184c5d8564e51904cf686a
Document Type :
article
Full Text :
https://doi.org/10.3390/foods12010196