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Detection and Quantification of the Epstein-Barr Virus in Lymphoma Patients from Ethiopia: Molecular and Serological Approaches

Authors :
Seifegebriel Teshome
Kidist Zealiyas
Abdulaziz Abubeker
Fisihatsion Tadesse
Jayalakshmi Balakrishna
Christoph Weigel
Tamrat Abebe
Elshafa Hassan Ahmed
Robert A. Baiocchi
Source :
Microorganisms, Vol 11, Iss 10, p 2606 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The Epstein-Barr virus (EBV) is a known oncogenic virus associated with various lymphoma subtypes throughout the world. However, there is a lack of information regarding EBV prevalence in lymphoma patients, specifically in Ethiopia. This study aimed to investigate the presence of the EBV and determine its viral load in lymphoma patients from Ethiopia using molecular and serological approaches. Lymphoma patient samples were collected from the Ethiopian population. DNA and serum samples were extracted and subjected to molecular detection methods, including quantitative polymerase chain reaction (qPCR) analysis targeting the EBNA1 gene. Serological analyses were performed using an enzyme-linked immunosorbent assay (ELISA) to detect EBV viral capsid antigen IgG antibodies. EBV DNA was detected in 99% of lymphoma patients using qPCR, and serological analyses showed EBV presence in 96% of cases. A high EBV viral load (>10,000 EBV copies/mL) was observed in 56.3% of patients. The presence of high EBV viral loads was observed in 59.3% of HL patients and 54.8% of NHL patients. This study provides important insights into the prevalence and viral load of the EBV among lymphoma patients in Ethiopia. The findings contribute to the limited knowledge in this area and can serve as a foundation for future research.

Details

Language :
English
ISSN :
20762607 and 07975597
Volume :
11
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.68ae079755974ea39aa54420f7bef0ab
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms11102606