Mechanisms of Shenghua Decoction on treating gynecological disease with blood deficiency and blood stasis syndrome explored by network pharmacology combined with molecular docking

Subject: This paper mainly studies the mechanism of action of Shenghua Decoction (SHD) combined with network pharmacology and molecular docking in the treatment of gynecological diseases with blood deficiency and blood stasis syndrome. In addition, we also verified the accuracy of the results of network pharmacology through related experiments. Method: Firstly, the clinical indications of Shenghua Decoction were analyzed based on bibliometrics. Targets of blood deficiency, blood stasis and dysmenorrhea were retrieved from MalaCards, PubMed and China National Knowledge Infrastructure (CNKI). The main active components and targets of SHD were obtained from Batman-TCM and TCMID. Then, the intersected targets of disease and SHD were obtained. A protein–protein interaction network was constructed, and the core targets were screened based on the topological analysis. GO and KEGG enrichment analysis were performed and a Herbs-Molecules-Targets network was established. Molecular docking technology was used to verify the affinity of main compounds to core protein targets. Finally, Elisa was conducted to quantify the target protein expression to verify the accuracy of prediction results. Results: CRP, PTGS2, TNF, IL6, INS, CAT an d IGF1 dominate the list of core targets for SHD of gynecologic disease based on network pharmacology method. In molecular function, SHD treatment of gynecologic diseases mainly involves regulation of biological quality (GO:0,065,008), trauma response (GO:0,009,611),etc. (FDR