Back to Search Start Over

Engineered probiotic Escherichia coli elicits immediate and long-term protection against influenza A virus in mice

Authors :
Ling Huang
Wei Tang
Lina He
Mengke Li
Xian Lin
Ao Hu
Xindi Huang
Zhouyu Wu
Zhiyong Wu
Shiyun Chen
Yangbo Hu
Source :
Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)
Publication Year :
2024
Publisher :
Nature Portfolio, 2024.

Abstract

Abstract Influenza virus infection remains a major global health problem and requires a universal vaccine with broad protection against different subtypes as well as a rapid-response vaccine to provide immediate protection in the event of an epidemic outbreak. Here, we show that intranasal administration of probiotic Escherichia coli Nissle 1917 activates innate immunity in the respiratory tract and provides immediate protection against influenza virus infection within 1 day. Based on this vehicle, a recombinant strain is engineered to express and secret five tandem repeats of the extracellular domain of matrix protein 2 from different influenza virus subtypes. Intranasal vaccination with this strain induces durable humoral and mucosal responses in the respiratory tract, and provides broad protection against the lethal challenge of divergent influenza viruses in female BALB/c mice. Our findings highlight a promising delivery platform for developing mucosal vaccines that provide immediate and sustained protection against respiratory pathogens.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.68693459a30349a7ae4eda1e5182e82c
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-024-51182-3