Back to Search
Start Over
Protocol for T-cell Adhesion Strength on Tumor Cells under Flow Conditions
- Source :
- Bio-Protocol, Vol 3, Iss 20 (2013)
- Publication Year :
- 2013
- Publisher :
- Bio-protocol LLC, 2013.
-
Abstract
- This method allows evaluating the relative adhesion strength between T lymphocytes and specific adherent target cells using a shear force in flow chambers. It is based on the measure of the resistance of conjugates formed between T cells and adherent tumor cells to shear stress in a microfluidic system. For this purpose, T cells, stained with a CellTracker probe, are added into flow channels containing a monolayer of adherent target cells and their progressive detachment under a constant shear stress is then recorded using a fluorescent microscope.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 23318325
- Volume :
- 3
- Issue :
- 20
- Database :
- Directory of Open Access Journals
- Journal :
- Bio-Protocol
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6743eca9216a49d9b9cc23b58bb839b6
- Document Type :
- article
- Full Text :
- https://doi.org/10.21769/BioProtoc.936