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Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy

Authors :
Davide Schiroli
Lucia Merolle
Eleonora Quartieri
Roberta Chicchi
Tommaso Fasano
Tiziana De Luca
Giuseppe Molinari
Stefano Pulcini
Thelma A. Pertinhez
Erminia Di Bartolomeo
Rino Biguzzi
Roberto Baricchi
Chiara Marraccini
Source :
Biomolecules, Vol 11, Iss 11, p 1638 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM patients periodically receive leucodepleted packed red blood cells (P-RBCs) stored for maximum 14 days. The aim of this study was to compare two alternative routine procedures to prepare the optimal P-RBCs product, in order to identify differences in their content that may somehow affect patients’ health and quality of life (QoL). In method 1, blood was leucodepleted and then separated to obtain P-RBCs, while in method 2 blood was separated and leucodepleted after removal of plasma and buffycoat. Forty blood donors were enrolled in two independent centers; couples of phenotypically matched whole blood units were pooled, divided in two identical bags and processed in parallel following the two methods. Biochemical properties, electrolytes and metabolic composition were tested after 2, 7 and 14 days of storage. Units prepared with both methods were confirmed to have all the requirements necessary for βTM transfusion therapy. Nevertheless, RBCs count and Hb content were found to be higher in method-1, while P-RBCs obtained with method 2 contained less K+, iron and storage lesions markers. Based on these results, both methods should be tested in a clinical perspective study to determine a possible reduction of transfusion-related complications, improving the QoL of βTM patients, which often need transfusions for the entire lifespan.

Details

Language :
English
ISSN :
2218273X
Volume :
11
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Biomolecules
Publication Type :
Academic Journal
Accession number :
edsdoj.66b231ec7441d8880c08434895b20
Document Type :
article
Full Text :
https://doi.org/10.3390/biom11111638