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Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest

Authors :
Marta Zarà
Jeness Campodonico
Nicola Cosentino
Maria Luisa Biondi
Patrizia Amadio
Gloria Milanesi
Emilio Assanelli
Silvia Cerri
Marco Biggiogera
Leonardo Sandrini
Calogero Claudio Tedesco
Fabrizio Veglia
Daniela Trabattoni
Fabio Blandini
Elena Tremoli
Giancarlo Marenzi
Silvia S. Barbieri
Source :
International Journal of Molecular Sciences, Vol 22, Iss 15, p 8065 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

The identification of new biomarkers allowing an early and more accurate characterization of patients with ST-segment elevation myocardial infarction (STEMI) is still needed, and exosomes represent an attractive diagnostic tool in this context. However, the characterization of their protein cargo in relation to cardiovascular clinical manifestation is still lacking. To this end, 35 STEMI patients (17 experiencing resuscitated out-of-hospital cardiac arrest (OHCA-STEMI) and 18 uncomplicated) and 32 patients with chronic coronary syndrome (CCS) were enrolled. Plasma exosomes were characterized by the nanoparticle tracking analysis and Western blotting. Exosomes from STEMI patients displayed a higher concentration and size and a greater expression of platelet (GPIIb) and vascular endothelial (VE-cadherin) markers, but a similar amount of cardiac troponin compared to CCS. In addition, a difference in exosome expression of acute-phase proteins (ceruloplasmin, transthyretin and fibronectin) between STEMI and CCS patients was found. GPIIb and brain-associated marker PLP1 accurately discriminated between OHCA and uncomplicated STEMI. In conclusion, the exosome profile of STEMI patients has peculiar features that differentiate it from that of CCS patients, reflecting the pathophysiological mechanisms involved in STEMI. Additionally, the exosome expression of brain- and platelet-specific markers might allow the identification of patients experiencing ischemic brain injury in STEMI.

Details

Language :
English
ISSN :
22158065, 14220067, and 16616596
Volume :
22
Issue :
15
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.65e7e993ae2c42508cf6da8f36207db0
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms22158065