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DEPLETION OF LYSYL OXIDASE-LIKE 4 (LOXL4) ATTENUATES COLONY FORMATION IN VITRO AND COLLAGEN DEPOSITION IN VIVO BREAST CANCER MODEL
- Source :
- Majalah Biomorfologi, Vol 34, Iss 2, Pp 67-73 (2024)
- Publication Year :
- 2024
- Publisher :
- Faculty of Medicine, Universitas Airlangga, 2024.
-
Abstract
- Highlights • Revealing LOX family members' mechanisms in promoting invasive breast cancer progression is essential for targeting specific molecules in invasive breast cancer. • Depletion of LOXL4 in invasive breast cancer shows attenuation of cell invasiveness in vitro and collagen deposition in tumor models in vivo. Abstract Background: Lysyl oxidase (LOX) family proteins have recently become a topic in cancer progression. Our recent study found a high expression of LOX-like 4 (LOXL4) in MDA-MB-231 cells. Objective: To reveal the impact of depleted LOXL4 in both in vitro and in vivo breast cancer models from a histological perspective. Material and Method: Endogenous LOXL4 was depleted using the CRISPR/Cas9 on MDA-MB-231 parental cells. Based on the LOXL4 protein expression, the clone was determined for the next experiment, thus generating MDA-MB-231 LOXL4 KO. Cell assay was conducted using colony formation assay (n=3) followed by crystal violet staining. The indicated cells were inoculated orthotopically to female BALB/c nude mice (n=5). At the end of the experiment, tumors were isolated, fixed, and prepared for Masson Trichrome staining. Result: CRISPR/Cas9 completely depleted LOXL4 expression on clone number #2-22. Depletion of LOXL4 reduced the colony size formed by MDA-MB-231 cells. MDA-MB-231 LOXL4 KO #2-22 derived tumors showed depressed tumor volume compared to the parental group. Reduced collagen was also observed from the Masson Trichrome staining (p
- Subjects :
- good health
lysyl oxidase
extracellular matrix
Biology (General)
QH301-705.5
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 02158833 and 27160920
- Volume :
- 34
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Majalah Biomorfologi
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.651d9482b74a44a0d5616ba61dbb32
- Document Type :
- article
- Full Text :
- https://doi.org/10.20473/mbiom.v34i2.2024.67-73