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Liquid condensation of reprogramming factor KLF4 with DNA provides a mechanism for chromatin organization

Authors :
Rajesh Sharma
Kyoung-Jae Choi
My Diem Quan
Sonum Sharma
Banumathi Sankaran
Hyekyung Park
Anel LaGrone
Jean J. Kim
Kevin R. MacKenzie
Allan Chris M. Ferreon
Choel Kim
Josephine C. Ferreon
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

KLF4, OCT4, SOX2 and MYC cooperate to reorganize chromatin during somatic cell reprogramming. Here the authors show that KLF4 forms a liquid-like biomolecular condensate that recruits OCT4 and SOX2, and that condensation of the isolated KLF4 DNA binding domain with DNA is enhanced by CpG methylation

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.64a6612989c448e0aa3b7aff1d6e39fe
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-25761-7