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An Oligonucleotide Microarray Resource for Transcriptional Profiling of Bradyrhizobium japonicum
- Source :
- Molecular Plant-Microbe Interactions, Vol 20, Iss 10, Pp 1298-1307 (2007)
- Publication Year :
- 2007
- Publisher :
- The American Phytopathological Society, 2007.
-
Abstract
- A DNA microarray, comprising 70-mer oligonucleotides, representing 8,453 open reading frames (ORFs), was constructed based on the Bradyrhizobium japonicum strain USDA110 genomic sequence. New annotation predicted 199 additional genes, which were added to the microarray and were shown to be transcribed. These arrays were used to profile transcription in cells under a variety of conditions, including growth in minimal versus rich medium, osmotic stress, and free-living cells versus bacteroids. Increased expression was seen for genes involved in translation, motility, and cell envelope synthesis in rich medium whereas expression increased in minimal medium for genes involved in vitamin biosynthesis and stress responses. Treatment with 50 mM NaCl activated stress-inducible genes but repressed genes involved in chemotaxis and motility. Strikingly, no known transport systems for accumulation of compatible solutes or osmoprotectants were induced in response to osmotic stress. A number of nif, fix, and hup genes, but not all, were upregulated in bacteroids. The B. japonicum type III secretion system, known to be important in early nodulation, was downregulated in bacteroids. The availability of a reliable, low-cost B. japonicum microarray provides a useful tool for functional genomic studies of one of the most agriculturally important bacteria.
- Subjects :
- transcriptomics
Microbiology
QR1-502
Botany
QK1-989
Subjects
Details
- Language :
- English
- ISSN :
- 19437706 and 08940282
- Volume :
- 20
- Issue :
- 10
- Database :
- Directory of Open Access Journals
- Journal :
- Molecular Plant-Microbe Interactions
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.63029d7299774885a082da6fd2e1e9c4
- Document Type :
- article
- Full Text :
- https://doi.org/10.1094/MPMI-20-10-1298