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Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme

Authors :
Jiangyu Yan
Thomas R. Beattie
Adriana L. Rojas
Kelly Schermerhorn
Tamzin Gristwood
Jonathan C. Trinidad
Sonja V. Albers
Pietro Roversi
Andrew F. Gardner
Nicola G. A. Abrescia
Stephen D. Bell
Source :
Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
Publication Year :
2017
Publisher :
Nature Portfolio, 2017.

Abstract

The current model for B-family DNA polymerases in archaea is one of single-subunit enzymes in contrast to the multi-subunit complexes in eukaryotes. Here the authors show that PolB1 fromSulfolobus solfataricusexists as a heterotrimeric complex in cell extracts.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
8
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.627c7d9ffa8c4d6aa1b8ab4ad90d0baa
Document Type :
article
Full Text :
https://doi.org/10.1038/ncomms15075