Histologic evaluation of vital pulp therapy using propolis and platelet-rich plasma (PRP): Evidence from a canine model

Objective: The present study evaluated the pulp tissue response following direct pulp capping (DPC) with several materials in dogs’ teeth.Methods: This study was performed on four doges with 64 mature healthy premolars and molars. The teeth randomly received the following materials: platelet-rich plasma (PRP), propolis, MTA, and glass-ionomer cement (GIC, negative control group). Afterwards, the teeth were restored with light cure GIC. Half of the animals were sacrificed after seven and half after 30 days. Histologic samples were prepared (n=8 per material/interval), and the levels of inflammation, and fibrous and calcified tissue formation were compared between the groups and intervals.Results: After seven days, inflammation and calcified tissue levels were comparable among the groups (P ). The MTA group showed significantly more fibrous tissue formation than the GIC samples (P0.05). Fibrous tissue formation in the MTA and propolis groups decreased significantly after 30 days (P