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Genome-Wide Identification and Expression Analysis of NPF Genes in Cucumber (Cucumis sativus L.)

Authors :
Mengying Zhang
Wenyan Zhang
Zijian Zheng
Zhiping Zhang
Bing Hua
Jiexia Liu
Minmin Miao
Source :
Plants, Vol 12, Iss 6, p 1252 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins perform an essential role in regulating plant nitrate absorption and distribution and in improving plant nitrogen use efficiency. In this study, cucumber (Cucumis sativus L.) NPF genes were comprehensively analyzed at the whole genome level, and 54 NPF genes were found to be unevenly distributed on seven chromosomes in the cucumber genome. The phylogenetic analysis showed that these genes could be divided into eight subfamilies. We renamed all CsNPF genes according to the international nomenclature, based on their homology with AtNPF genes. By surveying the expression profiles of CsNPF genes in various tissues, we found that CsNPF6.4 was specifically expressed in roots, indicating that CsNPF6.4 may play a role in N absorption; CsNPF6.3 was highly expressed in petioles, which may be related to NO3− storage in petioles; and CsNPF2.8 was highly expressed in fruits, which may promote NO3− transport to the embryos. We further examined their expression patterns under different abiotic stress and nitrogen conditions, and found that CsNPF7.2 and CsNPF7.3 responded to salt, cold, and low nitrogen stress. Taken together, our study lays a foundation for further exploration of the molecular and physiological functions of cucumber nitrate transporters.

Details

Language :
English
ISSN :
22237747
Volume :
12
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.620cfbb6c468442882dfdcf1910c9c1f
Document Type :
article
Full Text :
https://doi.org/10.3390/plants12061252