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Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells

Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells

Authors :
Benedetta Brugnoli
Alessia Mariano
Beatrice Simonis
Cecilia Bombelli
Simona Sennato
Antonella Piozzi
Vincenzo Taresco
Veeren M. Chauhan
Steven M. Howdle
Anna Scotto d'Abusco
Iolanda Francolini
Source :
Carbohydrate Polymer Technologies and Applications, Vol 6, Iss , Pp 100373- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Polymers are among the most studied materials as drug carriers, due to their tunable chemical structure and ability to self-assemble to give different types of nanostructures. In this study, chitosan (CS) nanoparticles (NPs) were investigated as carriers for the anticancer drug sodium usnate (NaU) for the treatment of osteosarcoma (OS), which is the most prevalent primary malignant bone sarcoma in pediatric and adolescent patients. CS nano-assembling was induced by electrostatic interactions with the drug and the anionic cross-linker tripolyphosphate, thus obtaining stable nanosystems and a high drug encapsulation efficiency. Importantly, a reduction in NaU hepatotoxicity when encapsulated in CS NPs compared to free NaU was evidenced, suggesting that CS may have a protective role against liver damage. Unfortunately, NaU encapsulation also reduced drug toxicity versus osteosarcoma 143B cells compared to free NaU. Nevertheless, NaU-loaded CS NPs (0.312 mg/mL) were found to decrease 143B cells viability after 48 h and 72 h treatment without being hepatotoxic. Interestingly, this system also stimulated in 143B cells Maspin production, an agent known for its tumor suppressor properties. Relevant synergistic activity between CS and NaU in promoting Maspin stimulation was found. That suggests the potential of the systems to reduce invasiveness of OS cancer.

Details

Language :
English
ISSN :
26668939 and 61556882
Volume :
6
Issue :
100373-
Database :
Directory of Open Access Journals
Journal :
Carbohydrate Polymer Technologies and Applications
Publication Type :
Academic Journal
Accession number :
edsdoj.615568822e44aa82585ff9873a9f38
Document Type :
article
Full Text :
https://doi.org/10.1016/j.carpta.2023.100373