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A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR)
- Source :
- Biology Open, Vol 7, Iss 6 (2018)
- Publication Year :
- 2018
- Publisher :
- The Company of Biologists, 2018.
-
Abstract
- The optomotor response (OMR) is a locomotor behavior of animals that is induced by moving repetitive visual stimuli. This characteristic helps animals particularly when stabilizing and maintaining position in schools and herds. Here, we developed a simple but sensitive method for quantifying the OMR using medaka (Oryzias latipes) as a model. This method, which simply requires video-recorded behavior, free tracking software, and a generic spreadsheet program, enables the evaluation of spectral sensitivity by researchers with little knowledge about the behavioral characteristics of the test animal or of the OMR. Based on a manual method, we reported previously that wild-type and red-colorblind medaka exhibited an OMR up to λ=830 and 740 nm, respectively. However, the present method, which quantifies the OMR according to three parameters (starting time, duration, and total distance of swimming) that are calculated based on a series of x–y coordinates of the moving fish, supported that conclusion and further indicated that both strains perceive light at even longer wavelengths. This low-cost, quantitative, and semi-automatic method would widen the opportunities to unveil behavioral photosensitivity in animals of interest. This article has an associated First Person interview with the first author of the paper.
Details
- Language :
- English
- ISSN :
- 20466390
- Volume :
- 7
- Issue :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Biology Open
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.614dcb1ba0ce4589a9ece48b8971b724
- Document Type :
- article
- Full Text :
- https://doi.org/10.1242/bio.033175