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Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of Enterococcus faecalis by PUI and XPF File Activation

Authors :
Alejandro Álvarez-Sagües
Nerea Herce
Ulises Amador
Francisco Llinares-Pinel
Estanislao Nistal-Villan
Jesús Presa
Laura Álvarez
Magdalena Azabal
Source :
Dentistry Journal, Vol 9, Iss 4, p 41 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Background: Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different activated irrigants using passive ultrasonic activation (PUI) or XP-Endo finisher (XPF). Methods: A total of 100 instrumented teeth were incubated in an Eppendorf tube containing 0.5 McFarland of Enterococcus faecalis and incubated for 2 weeks at 37 °C. Roots were divided into 5 groups (n = 20) according to the irrigant type: ethylenediaminetetraacetic acid (EDTA) (17%), ethydronic acid (HEDP) (9%) mixed with 5.25% sodium hypochlorite (NaOCl), EDTA (17%) mixed with 5.25% NaOCl, PBS, and a control group. Each group was divided into two subgroups (n = 10): PUI and XPF. Results: As compared to the untreated control group, the irrigators included in the study had a significant effect in bacteria reduction. The obtained results show HEDP to be the most effective irrigant, since no bacteria were recovered after treatment of this group, followed by EDTA mixed with NaOCl and, finally, the EDTA-irrigated group. Conclusions: HEDP is the best irrigating agent in combination with XPF or PUI file activation to eliminate bacteria in our experimental model.

Details

Language :
English
ISSN :
23046767
Volume :
9
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Dentistry Journal
Publication Type :
Academic Journal
Accession number :
edsdoj.6136b0e042bb43a2b9d96c35f4c80873
Document Type :
article
Full Text :
https://doi.org/10.3390/dj9040041