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PHENOTYPIC AND GENOTYPIC ANALYSIS OF A TRUEPERELLA BERNARDIAE STRAIN ISOLATED FROM A DOG
- Source :
- Veterinaria, Vol 66, Iss 2, Pp 66-71 (2017)
- Publication Year :
- 2017
- Publisher :
- University of Sarajevo, 2017.
-
Abstract
- The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog.
- Subjects :
- Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- Bosnian, English, Croatian, Serbian
- ISSN :
- 03726827 and 22331360
- Volume :
- 66
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- Veterinaria
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.60fa707f85c4ce6b5957b20c7ab0db3
- Document Type :
- article