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Protocol for preparing mammalian skin samples encompassing hair follicles for spatial transcriptomics
- Source :
- STAR Protocols, Vol 5, Iss 3, Pp 103254- (2024)
- Publication Year :
- 2024
- Publisher :
- Elsevier, 2024.
-
Abstract
- Summary: Spatial transcriptomics enables a single-cell resolution view of gene expression patterns in tissues, providing insight into their biological functions. However, applying this approach to the skin presents inherent challenges. Here, we present a protocol for preparing mammalian skin samples encompassing hair follicles for spatial transcriptomics. We describe steps for sample preparation, embedding, acquisition of frozen slices, RNA quality control, tissue mounting, fixation, staining, and imaging. We then detail procedures for permeabilization, reverse transcription, and cDNA collection.For complete details on the use and execution of this protocol, please refer to Chen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
- Subjects :
- Genetics
Genomics
Model Organisms
Molecular Biology
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 5
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.603ac9055bab4bfaa9375d89b8e59dfb
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2024.103254