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MOLECULAR DIAGNOSIS OF Guignardia citricarpa IN ASYMPTOMATIC SWEET ORANGE TISSUE

Authors :
FERNANDA DE SILLOS FAGANELLO
RENATO CARRER FILHO
VANESSA DUARTE DIAS
REGINA MELO SARTORI COELHO MORELLO
MARCOS GOMES DA CUNHA
Source :
Revista Brasileira de Fruticultura, Vol 39, Iss 4 (2017)
Publication Year :
2017
Publisher :
Sociedade Brasileira de Fruticultura, 2017.

Abstract

ABSTRACT Citrus black spot, a fungal disease caused by the quarantine fungus Guignardia citricarpa, restricts the exportation of fresh fruit to countries in the European Union. The occurrence of latent infections and the time required for diagnosis using conventional methods have brought about the need to validate fast, efficient and reproducible molecular techniques to detect the pathogen in asymptomatic tissue. As such, this study aims to detect G. citricarpa in the symptomatic fruit and asymptomatic leaf tissue of sweet oranges by conventional and real-time polymerase chain reaction (PCR). Specificity and limit of detection (LOD) were assessed in tissue samples of fruit lesions and asymptomatic leaves. Low concentrations of the fungus were found in asymptomatic leaves. Under these conditions, real-time PCR proved to be viable, reproducible and highly sensitive to detection of the pathogen.

Details

Language :
English, Spanish; Castilian, Portuguese
ISSN :
18069967 and 01002945
Volume :
39
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Revista Brasileira de Fruticultura
Publication Type :
Academic Journal
Accession number :
edsdoj.5ef8e436d6ee42b6bfe06b3852b9d447
Document Type :
article
Full Text :
https://doi.org/10.1590/0100-29452017518