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Anthocyanin-Rich Grape Pomace Extract (Vitis vinifera L.) from Wine Industry Affects Mitochondrial Bioenergetics and Glucose Metabolism in Human Hepatocarcinoma HepG2 Cells

Authors :
Nathalia F. F. de Sales
Leandro Silva da Costa
Talita I. A. Carneiro
Daniela A. Minuzzo
Felipe L. Oliveira
Lourdes M. C. Cabral
Alexandre G. Torres
Tatiana El-Bacha
Source :
Molecules, Vol 23, Iss 3, p 611 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Cancer cells demand high ATP provisions to support proliferation, and targeting of energy metabolism is a good strategy to increase their sensitivity to treatments. In Brazil, wine manufacture is expanding, increasing the amount of pomace that is produced. We determined the phenolic composition and antioxidant properties of a dark skin Grape Pomace Extract and its effects on metabolism and redox state in human hepatocarcinoma HepG2 cells. The material and the methods used represented the industrial process since pomace derived from white wine production and the extract concentrated by pilot plant scale reverse osmosis. Grape pomace extract was rich in polyphenols, mainly anthocyanins, and presented high antioxidant capacity. Short-term metabolic effects, irrespective of any cytotoxicity, involved increased mitochondrial respiration and antioxidant capacity and decreased glycolytic metabolism. Long-term incubation was cytotoxic and cells died by necrosis and GPE was not toxic to non-cancer human fibroblasts. To the best of our knowledge, this is the first report to characterize pomace extract from white wine production from Brazilian winemaking regarding its effects on energy metabolism, suggesting its potential use for pharmaceutical and nutraceutical purposes.

Details

Language :
English
ISSN :
14203049
Volume :
23
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Molecules
Publication Type :
Academic Journal
Accession number :
edsdoj.5eeac82c63de4bed9c6e522f2005dcc0
Document Type :
article
Full Text :
https://doi.org/10.3390/molecules23030611