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Characterisation of breast cancer molecular signature and treatment assessment with vibrational spectroscopy and chemometric approach.

Authors :
Magdalena Kołodziej
Ewa Kaznowska
Sylwia Paszek
Józef Cebulski
Edyta Barnaś
Marian Cholewa
Jitraporn Vongsvivut
Izabela Zawlik
Source :
PLoS ONE, Vol 17, Iss 3, p e0264347 (2022)
Publication Year :
2022
Publisher :
Public Library of Science (PLoS), 2022.

Abstract

Triple negative breast cancer (TNBC) is regarded as the most aggressive breast cancer subtype with poor overall survival and lack of targeted therapies, resulting in many patients with recurrent. The insight into the detailed biochemical composition of TNBC would help develop dedicated treatments. Thus, in this study Fourier Transform Infrared microspectroscopy combined with chemometrics and absorbance ratios investigation was employed to compare healthy controls with TNBC tissue before and after chemotherapy within the same patient. The primary spectral differences between control and cancer tissues were found in proteins, polysaccharides, and nucleic acids. Amide I/Amide II ratio decrease before and increase after chemotherapy, whereas DNA, RNA, and glycogen contents increase before and decrease after the treatment. The chemometric results revealed discriminatory features reflecting a clinical response scheme and proved the chemotherapy efficacy assessment with infrared spectroscopy is possible.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
19326203
Volume :
17
Issue :
3
Database :
Directory of Open Access Journals
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
edsdoj.5df068faa09d47b38afe2865a453e840
Document Type :
article
Full Text :
https://doi.org/10.1371/journal.pone.0264347