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Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age

Authors :
Leanne J. G. Chan
Niclas Olsson
Magdalena Preciado López
Kayley Hake
Haruna Tomono
Matthew A. Veras
Fiona E. McAllister
Source :
Proteomes, Vol 12, Iss 2, p 16 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

One of the main hallmarks of aging is aging-associated inflammation, also known as inflammaging. In this study, by comparing plasma and kidney proteome profiling of young and old mice using LC–MS profiling, we discovered that immunoglobulins are the proteins that exhibit the highest increase with age. This observation seems to have been disregarded because conventional proteome profiling experiments typically overlook the expression of high-abundance proteins or employ depletion methods to remove them before LC–MS analysis. We show that proteome profiling of immunoglobulins will likely be a useful biomarker of aging. Spatial profiling using immunofluorescence staining of kidney sections indicates that the main increases in immunoglobulins with age are localized in the glomeruli of the kidney. Using laser capture microdissection coupled with LC–MS, we show an increase in multiple immune-related proteins in glomeruli from aged mice. Increased deposition of immunoglobulins, immune complexes, and complement proteins in the kidney glomeruli may be a factor leading to reduced filtering capacity of the kidney with age. Therapeutic strategies to reduce the deposition of immunoglobulins in the kidney may be an attractive strategy for healthy aging.

Details

Language :
English
ISSN :
22277382
Volume :
12
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Proteomes
Publication Type :
Academic Journal
Accession number :
edsdoj.5db2b7496aa844578b6330033bfd77cb
Document Type :
article
Full Text :
https://doi.org/10.3390/proteomes12020016