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Microbe-metabolite interaction networks, antibiotic resistance, and in vitro reconstitution of the penile prosthesis biofilm support a paradigm shift from infection to colonization

Authors :
Glenn T. Werneburg
Scott D. Lundy
Daniel Hettel
Petar Bajic
Bradley C. Gill
Ava Adler
Sromona D. Mukherjee
Hadley M. Wood
Kenneth W. Angermeier
Daniel A. Shoskes
Aaron W. Miller
Source :
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Publication Year :
2023
Publisher :
Nature Portfolio, 2023.

Abstract

Abstract To understand differences between asymptomatic colonized and infected states of indwelling medical devices, we sought to determine penile prosthesis biofilm composition, microbe-metabolite interaction networks, and association with clinical factors. Patients scheduled for penile prosthesis removal/revision were included. Samples from swabbed devices and controls underwent next-generation sequencing, metabolomics, and culture-based assessments. Biofilm formation from device isolates was reconstituted in a continuous-flow stir tank bioreactor. 93% of 27 analyzed devices harbored demonstrable biofilm. Seven genera including Faecalibaculum and Jeotgalicoccus were more abundant in infected than uninfected device biofilms (p

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
20452322
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.5bfd8368e4424259b4c177c69b62c7e7
Document Type :
article
Full Text :
https://doi.org/10.1038/s41598-023-38750-1