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Exendin-4 Promotes Beta Cell Proliferation via PI3k/Akt Signalling Pathway
- Source :
- Cellular Physiology and Biochemistry, Vol 35, Iss 6, Pp 2223-2232 (2015)
- Publication Year :
- 2015
- Publisher :
- Cell Physiol Biochem Press GmbH & Co KG, 2015.
-
Abstract
- Background/Aims: Prevention of diabetes requires maintenance of a functional beta-cell mass, the postnatal growth of which depends on beta cell proliferation. Past studies have shown evidence of an effect of an incretin analogue, Exendin-4, in promoting beta cell proliferation, whereas the underlying molecular mechanisms are not completely understood. Methods: Here we studied the effects of Exendin-4 on beta cell proliferation in vitro and in vivo through analysing BrdU-incorporated beta cells. We also analysed the effects of Exendin-4 on beta cell mass in vivo, and on beta cell number in vitro. Then, we applied specific inhibitors of different signalling pathways and analysed their effects on Exendin-4-induced beta cell proliferation. Results: Exendin-4 increased beta cell proliferation in vitro and in vivo, resulting in significant increases in beta cell mass and beta cell number, respectively. Inhibition of PI3K/Akt signalling, but not inhibition of either ERK/MAPK pathway, or JNK pathway, significantly abolished the effects of Exendin-4 in promoting beta cell proliferation. Conclusion: Exendin-4 promotes beta cell proliferation via PI3k/Akt signaling pathway.
Details
- Language :
- English
- ISSN :
- 10158987 and 14219778
- Volume :
- 35
- Issue :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Cellular Physiology and Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.5bdad03e5f654336bcf7c6eafe3e44ab
- Document Type :
- article
- Full Text :
- https://doi.org/10.1159/000374027