Back to Search Start Over

Dexmedetomidine enhances tolerance to bupivacaine cardiotoxicity in the isolated rat hearts: alpha 2 adrenoceptors were not involved

Authors :
Fangfang Xia
Zhousheng Jin
Tingting Lin
Xixi Cai
Linmin Pan
Shi Wang
Yaoyao Cai
Hongfei Chen
Source :
BMC Pharmacology and Toxicology, Vol 20, Iss 1, Pp 1-8 (2019)
Publication Year :
2019
Publisher :
BMC, 2019.

Abstract

Abstract Background Dexmedetomidine was proved to mitigate bupivacaine-induced cardiotoxicity but mechanism of this ability is still unclear. This study was designed to investigate the direct effects of dexmedetomidine on cardiotoxicity induced by bupivacaine on Langendorff rat heart preparation and the role of alpha 2 adrenoceptors in this process was explored. Methods Hearts of rat were isolated, mounted on a Langendorff system. Five experimental groups were assessed after 10 min Krebs-Henseleit buffer (KHB) infusions as follow: (1) Group Con, only KHB was perfused; (2) Group Dex, KHB was perfused for 5 min, then dexmedetomidine (10 nmol/L) was added; (3) Group Bupi, KHB was perfused for 25 min, then bupivacaine (50 μmol/L) was added; (4) Group Bupi + Dex, KHB was perfused for 5 min, then the dexmedetomidine (10 nmol/L) was added for 20 min, at last a mixture of KHB + dexmedetomidine + bupivacaine were perfused; (5) Group Bupi + Dex + Yoh, a combination of KHB + yohimbine (alpha 2 adrenoceptor antagonists, 1 μmol/L) was perfusion for 5 min, then dexmedetomidine (10 nmol/L) was added for 20 min, at last a mixture of KHB + yohimbine + dexmedetomidine + bupivacaine was perfused. The experimental perfusion was maintained for 35 min in group Con and group Dex, and the experimental perfusion was sustained until asystole in the other three groups. Results Compared with group Bupi, dexmedetomidine significantly increased the time to first arrhythmia (P

Details

Language :
English
ISSN :
20506511
Volume :
20
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Pharmacology and Toxicology
Publication Type :
Academic Journal
Accession number :
edsdoj.5bc056c6a108425dacad3e3aeac2310e
Document Type :
article
Full Text :
https://doi.org/10.1186/s40360-019-0371-1