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Functional and transcriptomic characterization of carboplatin-resistant A2780 ovarian cancer cell line
- Source :
- Biological Research, Vol 52, Iss 1, Pp 1-13 (2019)
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Abstract Background Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen is based on carboplatin (CBDCA). However, CBDCA resistance is the main obstacle to a better prognosis. An in vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize cellular and molecular changes of induced CBDCA-resistant ovarian cancer cell line A2780. Methods The cell selection strategy used in this study was a dose-per-pulse method using a concentration of 100 μM for 2 h. Once 20 cycles of exposure to the drug were completed, the cell cultures showed a resistant phenotype. Then, the ovarian cancer cell line A2780 was grown with 100 μM of CBDCA (CBDCA-resistant cells) or without CBDCA (parental cells). After, a drug sensitivity assay, morphological analyses, cell death assays and a RNA-seq analysis were performed in CBDCA-resistant A2780 cells. Results Microscopy on both parental and CBDCA-resistant A2780 cells showed similar characteristics in morphology and F-actin distribution within cells. In cell-death assays, parental A2780 cells showed a significant increase in phosphatidylserine translocation and caspase-3/7 cleavage compared to CBDCA-resistant A2780 cells (P
Details
- Language :
- English
- ISSN :
- 07176287
- Volume :
- 52
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Biological Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.5b968cb14f094ad28b0adfe0083c92d5
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s40659-019-0220-0