Correlation analysis of muscle amino acid deposition and gut microbiota profile of broilers reared at different ambient temperatures

Objective Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood. Methods A total of 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium, and low temperature (HT, MT, and LT), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis. Results Our data showed that compared to the MT group, there was a decrease of muscle leucine and tyrosine (p