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Morphokinetic behavior of euploid and aneuploid embryos analyzed by time-lapse in embryoscope

Authors :
Deven V Patel
Preeti B Shah
Aditi P Kotdawala
Javier Herrero
Irene Rubio
Manish R Banker
Source :
Journal of Human Reproductive Sciences, Vol 9, Iss 2, Pp 112-118 (2016)
Publication Year :
2016
Publisher :
Wolters Kluwer Medknow Publications, 2016.

Abstract

BACKGROUND: Embryonic aneuploidy may result in miscarriage, implantation failure, or birth defects. Thus, it is clinically necessary to avoid the selection of aneuploid embryos during in vitrofertilization treatment. AIM: The aim of this study was to identify the morphokinetic differences by analyzing the development of euploid and aneuploid embryos using a time-lapse technology. We also checked the accuracy of a previously described model for selection of euploid embryos based on morphokinetics in our study population. MATERIALS AND METHODS: It is a retrospective study of 29 cycles undergoing preimplantation genetic screening from October 2013 to April 2015 at our center. Of 253 embryos, 167 suitable for biopsy embryos were analyzed for their chromosomal status using array-comparative genome hybridization (CGH). The morphokinetic behavior of these embryos was further analyzed in embryoscope using time-lapse technology. RESULTS: Among the analyzed embryos, 41 had normal and 126 had abnormal chromosome content. No significant difference in morphokinetics was found between euploid and aneuploid embryos. The percentage of embryos with blastulation was similar in the euploid (65.85%, 27/41) and aneuploid (60.31%, 76/126) embryos (P = 0.76). Although hard to define, majority of the chromosomal defects might be due to meiotic errors. On applying embryo selection model from Basile et al., embryos falling within optimal ranges for time to division to 5 cells (t5), time period of the third cell cycle (CC3), and time from 2 cell division to 5 cell division (t5-t2) exhibited greater proportion of normal embryos than those falling outside the optimal ranges (28.6%, 25.9%, and 26.7% vs. 17.5%, 20.8%, and 14.3%). CONCLUSION: Keeping a track of time interval between two stages can help us recognize aneuploid embryos at an earlier stage and prevent their selection of transfer. However, it cannot be used as a substitute for array CGH to select euploid embryos for transfer.

Details

Language :
English
ISSN :
09741208 and 19984766
Volume :
9
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Journal of Human Reproductive Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.58eb935922f2411c8008fc5d75948b02
Document Type :
article
Full Text :
https://doi.org/10.4103/0974-1208.183511