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Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei

Authors :
Ying Zou
Guosi Xie
Tianchang Jia
Tingting Xu
Chong Wang
Xiaoyuan Wan
Yingxia Li
Kun Luo
Xiaodong Bian
Xiuhua Wang
Jie Kong
Qingli Zhang
Source :
Pathogens, Vol 9, Iss 9, p 741 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

A new emerging disease called “translucent post-larvae disease” (TPD) or “glass post-larvae disease” (GPD) of Penaeus vannamei, characterized by pale or colorless hepatopancreas and digestive tract, has become an urgent threat to the shrimp farming industry. Following this clue that treatment of an antibacterial agent could alleviate the disease, systematic investigation of the potential infectious agent of TPD was conducted using bacterial identification and artificial challenge tests to fulfill Koch’s postulates. A dominant bacterial isolate, Vp-JS20200428004-2, from the moribund individuals was isolated and identified as Vibrio parahaemolyticus based on multi-locus sequence analysis. However, Vp-JS20200428004-2 differed from the V. parahaemolyticus that caused typical acute hepatopancreatic necrosis disease. Immersion challenge tests revealed that Vp-JS20200428004-2 could cause 100% mortality within 40 h at a dose of 1.83 × 106 CFU/mL, and experimental infected shrimp showed similar clinical signs of TPD. The Vp-JS20200428004-2 could be re-isolated and identified from the experimental infected individuals. Moreover, histopathological analysis of diseased samples indicated that Vp-JS20200428004-2 caused severe necrosis and sloughing of epithelial cells of the hepatopancreas and midgut in shrimp individuals both naturally and experimentally infected. Our present results indicated that Vp-JS20200428004-2 is a highly virulent infectious agent associated with the TPD and deserves further attention.

Details

Language :
English
ISSN :
20760817
Volume :
9
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Pathogens
Publication Type :
Academic Journal
Accession number :
edsdoj.58833d45e80b4cfe857d0eddc2f2faa0
Document Type :
article
Full Text :
https://doi.org/10.3390/pathogens9090741