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Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Authors :
Shoko Hasegawa
Takuya Tsutsumi
Shunsuke Fukushima
Yoshihiro Okabe
Junna Saito
Mina Katayama
Masato Shindo
Yusuke Yamada
Koichiro Shimomura
Kaori Yoneyama
Kohki Akiyama
Koh Aoki
Tohru Ariizumi
Hiroshi Ezura
Shinjiro Yamaguchi
Mikihisa Umehara
Source :
International Journal of Molecular Sciences, Vol 19, Iss 9, p 2645 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.

Details

Language :
English
ISSN :
14220067
Volume :
19
Issue :
9
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.5847c7f5bed496783f39c1262e12f07
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms19092645