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TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells

Authors :
Joon Bum Kim
Ji-Eun Bae
Na Yeon Park
Yong Hwan Kim
Seong Hyun Kim
Hyejin Hyung
Eunbyul Yeom
Dong Kyu Choi
Kwiwan Jeong
Dong-Hyung Cho
Source :
Current Issues in Molecular Biology, Vol 46, Iss 8, Pp 8658-8664 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Migrasomes, the newly discovered cellular organelles that form large vesicle-like structures on the retraction fibers of migrating cells, are thought to be involved in communication between neighboring cells, cellular content transfer, unwanted material shedding, and information integration. Although their formation has been described previously, the molecular mechanisms of migrasome biogenesis are largely unknown. Here, we developed a cell line that overexpresses GFP-tetraspanin4, enabling observation of migrasomes. To identify compounds that regulate migrasome activity in retinal pigment epithelial (RPE) cells, we screened a fecal chemical library and identified cadaverine, a biogenic amine, as a potent migrasome formation inducer. Compared with normal migrating cells, those treated with cadaverine had significantly more migrasomes. Putrescine, another biogenic amine, also increased migrasome formation. Trace amine-associated receptor 8 (TAAR8) depletion inhibited migrasome increase in cadaverine-treated RPE cells, and cadaverine also inhibited protein kinase A phosphorylation. In RPE cells, cadaverine triggers migrasome formation via a TAAR8-mediated protein kinase A signaling pathway.

Details

Language :
English
ISSN :
14673045 and 14673037
Volume :
46
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Current Issues in Molecular Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.580b3e78cf954cfeb2fa64c82ffb38a4
Document Type :
article
Full Text :
https://doi.org/10.3390/cimb46080510