Estrus response and fertility rate in Kundhi buffaloes following estrus synchronization in breeding season

The aim of the present study was to compare the effect of two estrus synchronization treatments i.e., Ovsynch alone and Ovsynch plus Controlled Internal Drug Release (CIDR), on the occurrence of estrus and conception rate in Kundhi buffalo during breeding season in Pakistan. Forty Kundhi buffaloes were randomly selected and were divided into three groups; Group A (n=16; Ovsynch) received 2 mL GnRH intramuscularly (i/m) on day 0 and 9. On day 7, 5 mL prostaglandin F2 and #945; (PGF2 and #945; analogue) was administered through i/m route. The buffaloes of Group B (n=17; Ovsynch+CIDR) received 2 mL GnRH on day 0 along with implantation of CIDR. On day 7, the CIDR was removed, and 5 mL PGF2 and #945; analogue was injected through i/m route. A second dose of GnRH was administered through i/m route after 48 h of PGF2 and #945; inj. in both groups. Group C (n=7; control) received 2 mL normal saline through i/m route on day 0, 7 and 9. The buffaloes of all three groups were artificially inseminated twice (12 h and 24 h after the second GnRH inj.) using frozen-thawed semen. Estrus response differed significantly (P