Back to Search Start Over

Limited Emergence of Salmonella enterica Serovar Infantis Variants with Reduced Phage Susceptibility in PhagoVet-Treated Broilers

Authors :
Sandra Sevilla-Navarro
Jennifer Otero
Júlia López-Pérez
Jan Torres-Boncompte
Tiago Prucha
Maarten De Gussem
Daniela Silva
Julia Burgan
Pablo Catalá-Gregori
Pilar Cortés
Montserrat Llagostera
Source :
Animals, Vol 14, Iss 16, p 2352 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Salmonella enterica serovar Infantis (S. Infantis) poses a growing issue in the poultry sector, with phage-based products emerging as a safe and effective control measure. This study investigated the emergence of reduced-phage-susceptibility variants (RPSV) of S. Infantis in PhagoVet-treated broilers, given that RPSV could undermine phage treatment efficacy. The bacteriophages in the PhagoVet product were characterized using transmission electron microscopy (TEM), genome sequencing, and infection profiling. Furthermore, two broiler trials were conducted: a challenge group (T1) and a challenge-and-treated group (T2). The S. Infantis infective dose was set at 104 and 106 colony-forming units (CFUs) per animal, with PhagoVet administration at 106 and 108 plaque-forming units (PFUs) per animal, in Trials 1 and 2, respectively. The results revealed that the four PhagoVet bacteriophages belonged to different genera. PhagoVet evidenced broad-spectrum efficacy against 271 strains representing 18 Salmonella serovars. In Trial 1, PhagoVet reduced bacterial counts in feces to nearly undetectable levels by day 42, with no RPSV detected. However, in Trial 2, three and five RPSVs were detected in feces and ceca, respectively. Consequently, PhagoVet demonstrated efficacy against S. Infantis in broilers, and the potential impact of RPSV is deemed unlikely to compromise its efficacy.

Details

Language :
English
ISSN :
20762615
Volume :
14
Issue :
16
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.56a23c5d21a74b1b9deadd627ee2ee71
Document Type :
article
Full Text :
https://doi.org/10.3390/ani14162352