Back to Search Start Over

An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser

Authors :
Dameli Assalauova
Young Yong Kim
Sergey Bobkov
Ruslan Khubbutdinov
Max Rose
Roberto Alvarez
Jakob Andreasson
Eugeniu Balaur
Alice Contreras
Hasan DeMirci
Luca Gelisio
Janos Hajdu
Mark S. Hunter
Ruslan P. Kurta
Haoyuan Li
Matthew McFadden
Reza Nazari
Peter Schwander
Anton Teslyuk
Peter Walter
P. Lourdu Xavier
Chun Hong Yoon
Sahba Zaare
Viacheslav A. Ilyin
Richard A. Kirian
Brenda G. Hogue
Andrew Aquila
Ivan A. Vartanyants
Source :
IUCrJ, Vol 7, Iss 6, Pp 1102-1113 (2020)
Publication Year :
2020
Publisher :
International Union of Crystallography, 2020.

Abstract

An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.

Details

Language :
English
ISSN :
20522525
Volume :
7
Issue :
6
Database :
Directory of Open Access Journals
Journal :
IUCrJ
Publication Type :
Academic Journal
Accession number :
edsdoj.5625da8d08d44edbb4f98276f17f6ada
Document Type :
article
Full Text :
https://doi.org/10.1107/S2052252520012798