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Isolation, purification, structural characteristic and antioxidative property of polysaccharides from A. cepa L. var. agrogatum Don
- Source :
- Food Science and Human Wellness, Vol 9, Iss 1, Pp 71-79 (2020)
- Publication Year :
- 2020
- Publisher :
- Tsinghua University Press, 2020.
-
Abstract
- Allium cepa L. var. agrogatum Don (ALAP) is commonly consumed in China as well as other regions and has various beneficial health effects. A novel acidic polysaccharide (named ALAP-21) was obtained from ALAP by ultrasonic and microwave-assisted extraction and purification using DEAE-52 anion exchange and Sephadex G-100 columns. The monosaccharide composition, structural and antioxidative properties of ALAP-21 were investigated by GC–MS chromatography, FT-IR and NMR spectroscopies and three antioxidative activity tests in vitro. The results showed that ALAP-21 was a heteropolysaccharide composed of glucose, galacturonic acid, mannose, galactose, arabinose, rhamnose, xylose, fructose and glucuronic acid with a relative molar ratio of 26.282: 27.546: 11.400: 4.781: 2.467: 2.445: 3.622: 1.106: 1.753, owning (1→4)-α-d-Glcp, (1→4)-α-d-GalAp6Me3OA, (1→4)-β-d-Galp6OMe, (1→2)-α-l-Rhap, (1→4)-β-d-Manp glycosidic linkages. (1→4)-α-d-Glcp and (1→4)-α-d-GalAp6Me3OA residues might be the main components of the sugar chain backbone of ALAP-21. Furthermore, ALAP-21 exhibited high potential for DPPH radicals (82.02%), hydroxyl radicals (53.33%) and superoxide anion radicals (50.28%). These results provide a reference for further research and rational development of ALAP polysaccharides. Keywords: Polysaccharides, Allium cepa L. var. agrogatum Don, Structural characterization, Antioxidative property
- Subjects :
- Nutrition. Foods and food supply
TX341-641
Subjects
Details
- Language :
- English
- ISSN :
- 22134530
- Volume :
- 9
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Food Science and Human Wellness
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.558316692924ee6ac01bc363fbeef7d
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.fshw.2019.12.006