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Novel cryopreservation medium for enhanced stability of T cells at −80°C

Authors :
WenXuan Zhou
Chen Wang
Yao Shi
Yi Pan
XiaDuo Meng
XunLei Kang
Xu Han
Source :
Frontiers in Hematology, Vol 3 (2024)
Publication Year :
2024
Publisher :
Frontiers Media S.A., 2024.

Abstract

The increasing demand for immune cell applications, both in clinical settings and in research laboratories, has highlighted the critical need for cryopreservation (banking) methods for T cells. While conventional techniques such as freezing with liquid nitrogen remain prevalent, they pose significant challenges including high equipment costs, safety considerations, and logistical hurdles in transportation. Our cryopreservation medium, C80EZ®, represents a novel approach, leveraging biocompatible polysaccharides as cryoprotectants to enable safe storage at −80°C. This paper presents a comprehensive series of tests assessing the effectiveness of C80EZ® in shielding T cells from the detrimental effects of cryopreservation. Importantly, our findings demonstrate that C80EZ® not only ensures the survival of T cells, with a particular emphasis on preserving the CD8+ subsets, but also maintains their critical function in targeting and eliminating cancer cells.

Details

Language :
English
ISSN :
28133935
Volume :
3
Database :
Directory of Open Access Journals
Journal :
Frontiers in Hematology
Publication Type :
Academic Journal
Accession number :
edsdoj.54b7cfff0dae4e4b98a76c68052bde78
Document Type :
article
Full Text :
https://doi.org/10.3389/frhem.2024.1346627