Back to Search Start Over

Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin

Authors :
Hashim Onn H
Lim Boon-Kiong
Abdullah-Soheimi Siti S
Shuib Adawiyah S
Source :
Proteome Science, Vol 8, Iss 1, p 58 (2010)
Publication Year :
2010
Publisher :
BMC, 2010.

Abstract

Abstract Background Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and screening purposes. In the present study, urine samples from patients with ovarian carcinoma were analysed by two-dimensional gel electrophoresis and the profiles generated were compared to those similarly obtained from age-matched cancer negative women. Results Significant reduced levels of CD59, kininogen-1 and a 39 kDa fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), and enhanced excretion of a 19 kDa fragment of albumin, were detected in the urine of patients with ovarian carcinoma compared to the control subjects. The different altered levels of the proteins were confirmed by Western blotting using antisera and a lectin that bind to the respective proteins. Conclusion CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma.

Subjects

Subjects :
Cytology
QH573-671

Details

Language :
English
ISSN :
14775956
Volume :
8
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Proteome Science
Publication Type :
Academic Journal
Accession number :
edsdoj.52fb334eda2b4a05bf7337b8a4ac90a6
Document Type :
article
Full Text :
https://doi.org/10.1186/1477-5956-8-58