Eco‐Friendly Production of 2D Ti3C2Tx MXene and Cytotoxicity Mitigation Toward Biomedical Applications

Abstract 2D MXenes find applications in several technology fields. Solution processing techniques can facilitate the integration of these materials within the technological supply chain. Here, a highly concentrated (up to 1.5 g L−1) and stable dispersion of 2D Ti3C2Tx MXene flakes is produced by an environmentally friendly liquid phase exfoliation process (LPE) in dihydrolevoglucosenone (Cyrene). The flakes preserve their elemental composition after 6 months, while those exfoliated in N‐Methyl‐2‐pyrrolidone (NMP), a commonly used solvent, undergo significant oxidation. The cytotoxicity of the MXene flakes in Cyrene and in NMP is investigated by assessing the cellular viability in a human keratinocyte (HaCaT) cell line, which is a relevant model for skin applications. The data show that the MXene flakes in Cyrene induce cytotoxic effects for a dose > 1 µg cm−2, as similarly observed for the MXene flakes in NMP. Efforts are made to overcome this by collecting the MXene flakes and redispersing them in a solution of bovine serum albumin (BSA). MXene in BSA does not affect cellular viability and cell morphology, possibly due to the formation of a protective protein corona around the individual MXene flakes. These findings illustrate an environmentally friendly strategy to produce biocompatible 2D MXene for biomedical applications.