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Identification and Genome Characterization of a Novel Virus within the Genus Totivirus from Chinese Bayberry (Myrica rubra)

Authors :
Zhongtian Xu
Yi’nan Gao
Kun Teng
Huoyang Ge
Xiaoqi Zhang
Mengjing Wu
Ruhui Li
Zujian Wu
Luping Zheng
Source :
Viruses, Vol 16, Iss 2, p 283 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Chinese bayberry (Myrica rubra) is an economically significant fruit tree native to eastern Asia and widely planted in south-central China. However, studies about the viruses infecting M. rubra remain largely lacking. In the present study, we employed the metatranscriptomic method to identify viruses in M. rubra leaves exhibiting yellowing and irregular margin symptoms collected in Fuzhou, a city located in China’s Fujian province in the year 2022. As a consequence, a novel member of the genus Totivirus was identified and tentatively named “Myrica rubra associated totivirus 1” (MRaTV1). The genome sequencing of MRaTV1 was determined by overlapping reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The two deduced proteins encoded by MRaTV1 have the highest amino acid (aa) sequence identity to the coat protein (CP) and RNA-dependent RNA polymerase (RdRP) of Panax notoginseng virus A (PNVA), a member of the genus Totivirus within the family Totiviridae, at 49.7% and 61.7%, respectively. According to the results of the phylogenetic tree and the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV) for the genus Totivirus, MRaTV1 is considered a new member of the genus Totivirus.

Details

Language :
English
ISSN :
19994915
Volume :
16
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.51f6ff15b68245afa0550d41055a57c8
Document Type :
article
Full Text :
https://doi.org/10.3390/v16020283