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Characterization of bovine respiratory syncytial virus isolated in Brazil

Authors :
C.W. Arns
J. Campalans
S.C.B. Costa
H.G. Domingues
R.C.F. D'Arce
R.S. Almeida
L.T. Coswig
Source :
Brazilian Journal of Medical and Biological Research, Vol 36, Iss 2, Pp 213-218 (2003)
Publication Year :
2003
Publisher :
Associação Brasileira de Divulgação Científica, 2003.

Abstract

This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and non-hemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a fragment of 481 bp corresponding to part of the gene that codes for protein F, whereas nRT-PCR-G amplified a fragment of 371 bp, in agreement with part of the G gene. The virus isolated from Brazilian samples in this study corresponded to the bovine respiratory syncytial virus, and RT-PCR proved to be useful for the diagnosis of bovine clinical samples.

Details

Language :
English
ISSN :
0100879X and 1414431X
Volume :
36
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Brazilian Journal of Medical and Biological Research
Publication Type :
Academic Journal
Accession number :
edsdoj.51c0c264d4e49768477d9b95029ef13
Document Type :
article
Full Text :
https://doi.org/10.1590/S0100-879X2003000200008