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Extra kinetic dimensions for label discrimination

Authors :
Raja Chouket
Agnès Pellissier-Tanon
Aliénor Lahlou
Ruikang Zhang
Diana Kim
Marie-Aude Plamont
Mingshu Zhang
Xi Zhang
Pingyong Xu
Nicolas Desprat
Dominique Bourgeois
Agathe Espagne
Annie Lemarchand
Thomas Le Saux
Ludovic Jullien
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

Label discrimination is challenging in fluorescence microscopy due to broad spectra and narrow lifetime distribution. Here, the authors introduce extra kinetic dimensions by illuminating reversibly photoswitchable fluorophores with different intensities, and discriminate 20 spectrally similar fluorophores.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.517de5688a41b097f0101dd109c7be
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-29172-0