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GNAI1 Suppresses Tumor Cell Migration and Invasion and is Post-Transcriptionally Regulated by Mir-320a/c/d in Hepatocellular Carcinoma

Authors :
Jian Yao
Lin-hui Liang
Yu Zhang
Jie Ding
Qi Tian
Jin-jun Li
Xiang-huo He
Source :
Cancer Biology & Medicine, Vol 9, Iss 4, Pp 234-241 (2012)
Publication Year :
2012
Publisher :
China Anti-Cancer Association, 2012.

Abstract

Objective To explore the role and regulation of guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1) in hepatocellular carcinoma (HCC).Methods Expression of GNAI1 in HCC samples was determined by qRT–PCR and immunohistochemical (IHC) staining. Huh-7 and SNU-387 cells stably expressing GNAI1 were established by the infection of lentivirus transducing unit containing GNAI1. siRNA against GNAI1 was transfected into SMMC-7721 cells to knock down the GNAI1 expression in HCC cells. Mir-320a/c/d mimics were transfected into SMMC-7721 and SK-Hep-1 cells and the expression of GNAI1 was determined by Western blot. The migration and invasion of Huh-7, SNU-387, SK-Hep-1 and SMMC-7721 cells were investigated by Transwell assays.Results The GNAI1 protein was significantly downregulated in HCC samples without changes in its mRNA levels. GNAI1 could inhibit the migration and invasion of HCC cells in vitro. Further investigations indicated that GNAI1 was a target of miR-320a/c/d in HCC cells. Transwell assays demonstrated that these microRNAs could promote the migratory ability and invasivesess of HCC cells in vitro.Conclusions GNAI1 is downregulated in HCC and inhibits the migration and invasion of HCC cells. This study is the first to investigate the role of GNAI1 in cancer. Regulation of GNAI1 by miR-320a/c/d indicates new therapeutic avenues for targeting HCC metastasis.

Details

Language :
English
ISSN :
20953941
Volume :
9
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Cancer Biology & Medicine
Publication Type :
Academic Journal
Accession number :
edsdoj.5119f90245e24c368d0bdf8f4327f2d0
Document Type :
article
Full Text :
https://doi.org/10.3969/j.issn.2095-3941.2012.04.003