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Organizing Multi-Enzyme Systems into Programmable Materials for Biocatalysis

Authors :
Min-Ju Seo
Claudia Schmidt-Dannert
Source :
Catalysts, Vol 11, Iss 4, p 409 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Significant advances in enzyme discovery, protein and reaction engineering have transformed biocatalysis into a viable technology for the industrial scale manufacturing of chemicals. Multi-enzyme catalysis has emerged as a new frontier for the synthesis of complex chemicals. However, the in vitro operation of multiple enzymes simultaneously in one vessel poses challenges that require new strategies for increasing the operational performance of enzymatic cascade reactions. Chief among those strategies is enzyme co-immobilization. This review will explore how advances in synthetic biology and protein engineering have led to bioinspired co-localization strategies for the scaffolding and compartmentalization of enzymes. Emphasis will be placed on genetically encoded co-localization mechanisms as platforms for future autonomously self-organizing biocatalytic systems. Such genetically programmable systems could be produced by cell factories or emerging cell-free systems. Challenges and opportunities towards self-assembling, multifunctional biocatalytic materials will be discussed.

Details

Language :
English
ISSN :
20734344
Volume :
11
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Catalysts
Publication Type :
Academic Journal
Accession number :
edsdoj.50a9a4983a3e43b3ab8c082ee0581c45
Document Type :
article
Full Text :
https://doi.org/10.3390/catal11040409