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A multicenter, randomized study of decitabine as epigenetic priming with induction chemotherapy in children with AML

Authors :
Lia Gore
Timothy J. Triche
Jason E. Farrar
Daniel Wai
Christophe Legendre
Gerald C. Gooden
Winnie S. Liang
John Carpten
David Lee
Frank Alvaro
Margaret E. Macy
Carola Arndt
Philip Barnette
Todd Cooper
Laura Martin
Aru Narendran
Jessica Pollard
Soheil Meshinchi
Jessica Boklan
Robert J. Arceci
Bodour Salhia
Source :
Clinical Epigenetics, Vol 9, Iss 1, Pp 1-13 (2017)
Publication Year :
2017
Publisher :
BMC, 2017.

Abstract

Abstract Background Decitabine is a deoxycytidine nucleoside derivative inhibitor of DNA-methyltransferases, which has been studied extensively and is approved for myelodysplastic syndrome in adults but with less focus in children. Accordingly, we conducted a phase 1 multicenter, randomized, open-label study to evaluate decitabine pre-treatment before standard induction therapy in children with newly diagnosed AML to assess safety and tolerability and explore a number of biologic endpoints. Results Twenty-four patients were fully assessable for all study objectives per protocol (10 in Arm A = epigenetic priming induction, 14 in Arm B = standard induction). All patients experienced neutropenia and thrombocytopenia. The most common grade 3 and 4 non-hematologic adverse events observed were gastrointestinal toxicities and hypophosphatemia. Plasma decitabine PK were similar to previously reported adult data. Overall CR/CRi was similar for the two arms. MRD negativity at end-induction was 85% in Arm A versus 67% in Arm B patients. DNA methylation measured in peripheral blood over the course of treatment tracked with blast clearance and matched marrow aspirates at day 0 and day 21. Unlike end-induction marrow analyses, promoter methylation in blood identified an apparent reversal of response in the lone treatment failure, 1 week prior to the patient’s marrow aspirate confirming non-response. Decitabine-induced effects on end-induction (day 35–43 following initiation of treatment) marrows in Arm A were reflected by changes in DNA methylation in matched paired marrow diagnostic aspirates. Conclusions This first-in-pediatrics trial demonstrates that decitabine prior to standard combination chemotherapy is feasible and well tolerated in children with newly diagnosed AML. Pre-treatment with decitabine may represent a newer therapeutic option for pediatric AML, especially as it appears to induce important epigenetic alterations. The novel biological correlates studied in this trial offer a clinically relevant window into disease progression and remission. Additional studies are needed to definitively assess whether decitabine can enhance durability responses in children with AML. Trial registration NCT01177540

Details

Language :
English
ISSN :
18687075 and 18687083
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Clinical Epigenetics
Publication Type :
Academic Journal
Accession number :
edsdoj.503b27da154bdf96868461841e32fb
Document Type :
article
Full Text :
https://doi.org/10.1186/s13148-017-0411-x