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Transcriptome Analysis of Human Glioblastoma Cells Susceptible to Infection with the Leningrad-16 Vaccine Strain of Measles Virus

Authors :
Yulia Ammour
Olga Susova
George Krasnov
Eugenia Nikolaeva
Vyacheslav Varachev
Yulia Schetinina
Marina Gavrilova
Alexey Mitrofanov
Anna Poletaeva
Ali Bekyashev
Evgeny Faizuloev
Vitaly V. Zverev
Oxana A. Svitich
Tatiana V. Nasedkina
Source :
Viruses, Vol 14, Iss 11, p 2433 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Glioblastoma multiforme (GBM) accounts for almost half of all primary malignant brain tumors in adults and has a poor prognosis. Here we demonstrated the oncolytic potential of the L-16 vaccine strain of measles virus (MV) against primary human GBM cells and characterized the genetic patterns that determine the sensitivity of primary human GBM cells to oncolytic therapy. MV replicated in all GBM cells, and seven out of eight cell lines underwent complete or partial oncolysis. RNA-Seq analysis identified about 1200 differentially expressed genes (FDR < 0.05) with at least two-fold expression level change between MV-infected and uninfected cells. Among them, the most significant upregulation was observed for interferon response, apoptosis and cytokine signaling. One out of eight GBM cell lines was defective in type I interferon production and, thus, in the post-interferon response, other cells lacked expression of different cellular defense factors. Thus, none of the cell lines displayed induction of the total gene set necessary for effective inhibition of MV replication. In the resistant cells, we detected aberrant expression of metalloproteinase genes, particularly MMP3. Thus, such genes could be considered intriguing candidates for further study of factors responsible for cell sensitivity and resistance to L-16 MV infection.

Details

Language :
English
ISSN :
19994915
Volume :
14
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.503a91ea0db4e8d9b2b168b7b45b4f5
Document Type :
article
Full Text :
https://doi.org/10.3390/v14112433