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Characterization of Grapevine Fanleaf Virus Isolates in ‘Chardonnay’ Vines Exhibiting Severe and Mild Symptoms in Two Vineyards

Authors :
Julie Kubina
Jean-Michel Hily
Pierre Mustin
Véronique Komar
Shahinez Garcia
Isabelle Rachel Martin
Nils Poulicard
Amandine Velt
Véronique Bonnet
Laurence Mercier
Olivier Lemaire
Emmanuelle Vigne
Source :
Viruses, Vol 14, Iss 10, p 2303 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Fanleaf degeneration is a complex viral disease of Vitis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. In France, its main causal agent is grapevine fanleaf virus (GFLV). In the past, field experiments were conducted to explore cross-protection as a management strategy of fanleaf degeneration, but results were unsatisfactory because the mild virus strain negatively impacted fruit yield. In order to select new mild GFLV isolates, we examined two old ‘Chardonnay’ parcels harbouring vines with distinct phenotypes. Symptoms and agronomic performances were monitored over the four-year study on 21 individual vines that were classified into three categories: asymptomatic GFLV-free vines, GFLV-infected vines severely diseased and GFLV-infected vines displaying mild symptoms. The complete coding genomic sequences of GFLV isolates in infected vines was determined by high-throughput sequencing. Most grapevines were infected with multiple genetically divergent variants. While no specific molecular features were apparent for GFLV isolates from vines displaying mild symptoms, a genetic differentiation of GFLV populations depending on the vineyard parcel was observed. The mild symptomatic grapevines identified during this study were established in a greenhouse to recover GFLV variants of potential interest for cross-protection studies.

Details

Language :
English
ISSN :
19994915
Volume :
14
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.4ff136741d2a4230a5fe55d9817351d3
Document Type :
article
Full Text :
https://doi.org/10.3390/v14102303